Product Description
YAP Rabbit monoclonal antibody is available at gentaur for next week delivery
Background: The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.
Applications: WB ICC/IF IHC FC
Purification&Purity: Protein A affinity purified
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of YAP and does not cross-react with related proteins.
W4BiowMW: ~ 67 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant antibody.Alternative Name:
Yorkie homolog; 65 kDa Yes-associated protein; YAP1; YAP65Western blot analysis: Western blot (WB) analysis of YAP Rabbit mAb at 1:1000 dilution Lane1:HepG2 whole cell lysate
Immunohistochemistry: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YAP antibody. Counter stained with hematoxylin.
Immunofluorescence analysis: ICC staining YAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Host: Rabbit
Swiss-Prot: P46937
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