sodium potassium ATPase alpha 1 subunit) from RDI Divison
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mouse anti-Na+/K+ alpha 1 subunit Monoclonal ab (M7PBE9)
cat# RDI-atpa1M7abm $469.00/vial
Background: The NA+/K+ Atpase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of NA and K across the cell membrane. The enzyme consists of a large multipass transmembrane catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta subunit). Studies indicate that there are three isoforms of the alpha subunit (A1, a2, & A3) and two isoforms of the Beta subunit (B1 & B2) which are encoded by two multigene families. The alpha 3 subunit has been found in neuronal and to a lesser extent skeletal and smooth muscle.
isotype: mIgG1
clone: M7-PB-E9
package: 200ul of diluted ascites fluid with 0.05% NaN3
antigen: produced from a fusion of SP20/0-Ag14 myeloma cells and spleen cells from F1 hybrid mice immunized with purified SHEEP kidney alpha subunit Na+ K+ Atpase.
Specificity: alpha 1 subunit of NA+/K+ ATPase -in human, sheep, canine , chicken and pig. (also sees alpha 3 subunit in rat but not alpha 1)
-binds to region between amino acids 646 and 652 of the sheep kidney NA K Atpase alpha subunit.
Use: -western blot (detects a band at approx 110kDa) 1:250
-immunoprecipitation
-Immunohistochemistry: 1:100
ref: -W.J. Ball, et al, Biochimica et Biophysica Acta, 719:413-423, 1982.
-A. Abbott and W.J. Ball, Biochemistry, 32:3511-3519, 1993
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.