CYTOKERATIN ANTIBODIES
CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
ANTI-CYTOKERATIN TYPE I & II
Mab to Cytokeratin Type I & II cat#RDI-PRO61835 $438.00/vial
Clone Determination AE 1 / AE 3
Category Mouse biclonal
Immunoglobulin Class IgG1
Purification Ion-exchange chromatography
Antigen Human epidermal keratin
Specificity AE 1/AE 3 represents an excellent marker with a very broad
range; for distinguishing Ca. from non-epithelial tumors
Application Suitable for · frozen and paraffin-embedded tissue (P)
· cytological material · immunoblotting (Western)
Storage At 4°C one year
Quantity 200 µl (1 mg/ml)
Polypeptide Reacting MR 40 000, MR 48 000 and MR 50 000 polypeptides of human epithelial proteins (cytokeratins nos. 19, 16, 14) and most other acidic (type I) cytokeratins (AE1). Most basic (type II) cytokeratins (MR 52 500 - 68 000) of human epithelia (AE3)
Antigen Recognized in
Species (tested so far) Human, rabbit, mouse, rat, bovine, chick, monkey, guinea pig
Reactivities on Cultured
Cell Lines (tested so far) MCF-7, A-431, RT 112, HeLa
Tumors Specifically Detected All epithelium and their neoplasms
Pretreatment With paraffin-embedded sections, protease pretreatment is recommended prior to antibody application
Working Dilution Dilute 1:200 in PBS pH 7.4 for use in immunohistochemistry Incubation Time 2h at 37°C (or over night at 4°C)