Immuno

(mouse anti-Beta Tubulin) CLASS III CLONE TUJ1

Catalog#: RDI-tubulB1abmX   $450.00/vial

Package Size: 100ug in 0.1ml (1mg/ml) in PBS with 5% trehalose

lot#

clone: TUJ-1

Ig Isotype: mouse IgG2a (Protein G purified from cell culture)

Antigen: rat brain microtubules

Species: Broad mammalian reactivity

Reactivity: Highly reactive with neuron specific CLASS III beta tubulin (III) and does not identify beta tubulin found in glial cells. Clone TUJ1 has been used successfully in immunoblotting (1:1000), predicted MW 50kda. immunoprecipitation and immunohistochemistry/immunofluorescence (1:100)

-This antibody can be used with the appropriate secondary reagents at the concentration of 510ug/ml in fixed cells. Cells were fixed with 4% paraformaldehyde and 0.15% picric acid in PBS at room temperature for 20 min., followed by blocking and permeablized with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA at room temperature for 45 min. After blocking, cells were incubated with diluted primary antibody overnight at 4 � C and followed by incubation for 1 hour with appropriate secondary antibody at room temperature. Between each step, cells were washed with PBS and 0.1% BSA.

Storage: Antibody can be aliquotted and stored frozen at -20 � months without detectable loss of activi ty. The without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

ANTIBODY TO:  HUMAN �-III ISOFORM OF TUBULIN (human/mouse/rat/bovine++)

PRODUCT CODE   RDI-CBL412   $375.00/set 2 vials 100ug each

CLONE           TU-20

ISOTYPE        IgG1

SOURCE         Mouse

PURIFICATION METHOD DEAE ion exchange chromotography

SPECIFICITY     This antibody reacts with the �-III isoform of tubulin C-peptide, amino acid sequence ESESQGPK, which is known to be neuron specific. Epitope C terminus

APPLICATIONS The antibody has application in:

*Immunoblotting (50kda band)

*Immunohistochemical staining of frozen sections

*Immunohistochemical staining of formalin fixed paraffin embedded tissue sections (recommend citrate buffer antigen release)

KNOWN SPECIESCROSS REACTIVITY Cross reacts with murine neuronal tissue    Also reacts with bovine,human,monkey,mouse, pig and rat (others not tested)

Ref:Dr�berov� E., Lukas Z., Ivanyi D., Viklicky V., Draber P. Expression of class III beta-tubulin in normal and neoplastic human tissues. Histochem. Cell Biol. 109: 231-239, 1998.

PRESENTATION The monoclonal is presented at a concentration of 100�g/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.