Glycophorin Antibodies

CD235A (Glycophorin A) Antibodies  

MOUSE MONOCLONAL ANTIBODY TO: HUMAN GLYCOPHORIN A (EXTRACELLULAR DOMAIN)

PRODUCT CODE        RDI-CBL409      $375.00/vial 200ug

Also available FITC conjugated   cat#RDI-CBL409FT $375.00/100T

                            PE conjugated:  cat#RDI-CBL409PE $469.00/100T

CLONE                         BRIC 256

ISOTYPE                      IgG1

SOURCE                      Tissue culture supernatent

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY The antibody reacts with a sialoglycoprotein present on human red blood cells and embryoid precursors. Mature, non-nucleated red cells are glycophorin A positive whilst being CD45 and CD71 negative.

APPLICATIONS

* Studies of glycoprotein A in erythroid development.* As a positive control of red blood cells in PNH studies in flow cytometry

REFERENCES   Mushens R & Scott M. (1990) J. Immunol. Meth. 131: 83-89

PRESENTATIONRDI-CBL409 CONT'D The monoclonal is presented as a 2 X 100ug (200ug) vial in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available FITC conjugated cat#RDI-CBL409FT $375.00/100T

                            PE conjugated: cat#RDI-CBL409PE $469.00/100T

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Product Specification: mouse monoclonal anti-human erythrocytes

CAT# RDI-M1365clb    PRICE: $375.00

Code: CLB-Ery/1

Lot Number:

Tests/vial: 200|

Specificity:

Cell type: Human erythrocytes.

Ag.MW.: 43kD.

Reactivity: The monoclonal antibody is directed against the alpha-chain of glycophorin. In indirect agglutination tests this monoclonal antibody reacts with normoblasts and erythrocytes. No reaction occurs with En(a-) erythrocytes and other normal peripheral blood cells.

Application: The monoclonal antibody is very useful for the recognition of malignant erythroblastic diseases.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-143 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human erythrocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Ascites fluid of tumor bearing BALB/c mice.

Purirfication:Ammoniumsulphate precipitation and ion exchange chromatography, followed by affinity chromatography

Packing: Each vial contains 1 ml with approximately 0.1mg/ml monoclonal antibody and 10 mg BSA in 20mM TRIS and 150 mM NaCl, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18 Deg C. Repeated thawing and freezing may reduce the antibody activity.

For IN Vitro Research Use Only-not for use in daignostics

Product Specification: mouse monoclonal anti-human erythrocytes FITC conjugated

CAT#: RDI-M1593clb   PRICE: $375.00

Code: CLB-Ery/1-F (CLB-AME-1)

Lot Number:

Specificity:

Cell type: Human erythrocytes.

Ag.MW.: 43 kD.

Reactivity: The monoclonal antibody is directed against the alpha chain of glycophorin. In indirect agglutination tests this monoclonal antibody reacts wtih normoblasts and erythrocytes. No reaction occurs with En(a-) erythrocytes and other normal peripheral blood cells.

Application: The monoclonal antibody is very useful for the recognition of malignant erythroblastic diseases.

Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-143  This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human erythrocytes.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Ascites fluid of tumor bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Chemical Characteristics:

Molecular F/P ratio : 8.2

Weight F/P ratio :21.2x10-3

Fluorescein concentration : 1.06x10-3 mg/ml

Optical density ratio : 1.34 (E495/E280) at pH 7.2

Packing: Each vial contains 1 ml with approximately 50ug/ml monoclonal antibody and 10 mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be 4-8 DEG C.