Recombinant Proteins

Mab to Glial Filament Protein

cat#RDI-PRO61011    $375.00/vial    BULK ON REQUEST

Clone Determination GF 12.24

Category Mouse monoclonal

Immunoglobulin Class IgG2a

Purification SpAss-Protein A (recombinant)

Antigen Intermediate filament cytoskeleton from cultured human glioma cells

Specificity GF 12.24 represents an excellent marker for cell typing; prenatal diagnosis of neural tube defects

Application Suitable for frozen and paraffin- embedded tissue (P)

Cytological material

Immunoblotting (Western)

Species: Human, bovine, rat and mouse

Stability at 4°C one year after reconstitution

Quantity 50 µg (lyoph.) (with BSA and azide) Bulk on request

For In Vitro Research Use Only

Pab to Glial Fibrillary Acidic Protein

cat#RDI-PRO10555    $312.00/0/.25ml vial

Category Rabbit polyclonal

Purification/Form Rabbit serum

Immunogen Isolated from human spinal cord.

Clinical Application/ Specificity The antibody is directed against the 56 kDa GFAP protein, the main subunit of intermediate filaments of glial cells and astrocytes. The antibody can be used to discriminate glial tumors (astrocytomas, ependymonas) from other tumors.

Application · Immunohistochemistry on frozen and paraffin-embedded tissue sections.

                       (Proteolytic treatment not required)

                     · Immunoblotting

Antigen Recognized in species(tested so far)  Human, cow, rat

Recommended Positive Control Brain tissue

Working Dilution Dilute 1:50; optimal dilution should be tested with serial dilution.

Dilution Buffer 0.15 M Phosphate buffered saline with 1% BSA and 0.1% Na-Azide

Storage/Stability At 4°C for min. 1 year

Quantity 0.25 ml; 0.09% NaN3 as preservative

Reference:

Perentes, E. et al. Recent Applications of Immunoperoxidase Histochemistry in Human Neuro-Oncology (1987). Archiv Path. Lab. Med. 111:796-812

Mouse anti-GFAP (moncolonal cocktail)

-cat#RDI-GFAPabm-CK   $562.00/0.5mg

-clones 4A11, 1B4 and 2E1 (equal protions)

-all mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells

and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue ections

-immunogen: bovine spinal cord homogenate

-note: by use of a cocktail, gerater overall sensitivity may be seen in histochemistry

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)

Mouse anti-GFAP (clone 4A11)

-cat#RDI-GFAPabm-411   $469.00/0.5mg

-clones 4A11

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)

Mouse anti-GFAP (clone 1B4)

-cat#RDI-GFAPabm-1B4   $469.00/0.5mg

-clones 1B4

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate

-ref: Neurochem Pathol. 3:119-138 (1985)

-ref: J Neuropath Exp Neuro 45:692-703 (1986)

Mouse anti-GFAP (clone 2E1

-cat#RDI-GFAPabm-2E1   $469.00/0.5mg

-clones 2E1

-mouse IgG2b

-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken

-use: western blot, indirect immunofluorescence of tissue cultured cells

and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections

-immunogen: bovine spinal cord homogenate