Cytoskeletal proteins

ANTI-CYTOKERATIN 7

Mab to Cytokeratin 7     cat#RDI-PRO61025   $375.00/50ug

Clone Determination                   Ks 7.18

Category                                    Mouse monoclonal

Immunoglobulin Class                 IgG1

Purification                                 SpAss Protein A (recombinant)

Antigen                                      Cytoskeletal proteins from cultured HeLa cells

Special Note                             Ks 7.18 represents an excellent marker for the discrimination of specific

                                                 subtypes of    adenocarcinoma: Eg adeno-CA of pancreas, bile duct

                                                 CA and transitional CA of bladder are positively stained, whereas colorectal,

                                                 hepatocellular and prostate CA are negative

Application                               Suitable for frozen and paraffin embedded tissue Cytological material

Stability/Storage                      One year after reconstitution when stored at 4°C

Quantity                                 50µg (lyoph.)

Polypeptide Reacting             MR 54 000 polypeptide (cytokeratin no.7) of human glandular epithelia

Antigen Recognized in Species

(tested so far)                        Human, bovine, pig, sheep; not with dog, rabbit, rat, mouse

Reactivities on Cultured

Cell Lines (tested so far)       HeLa, RT 112, T24, BT-20, CAMA-1 Detroit 562, (MCF-7, HT-29, are negative)

Tumors Specifically Detected Specific subtypes of adeno-CA: Adeno-CA of pancreas, gallbladder, lung, cervix; cholangio-CA of liver; ductal and lobular CA of breast; CA of ovary; transitional cell CA of bladder; mesothelioma; negative with colorectal Adeno CA and most cases of hepatocellular CA. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.

Application Suitable for frozen and paraffin (P) embedded tissue ,Cytological material Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application Working Dilution When reconstituted with 1ml destilled water, dilute 1:50 (at least) with PBS, pH 7.4 for use in immunohistochemistry Incubation Time 1h at RT, extended with P (over night at 4°C)