Mab to Prostate Specific Antigen (PSA)
Mab to Prostate Specific Antigen (PSA)
Category Mouse monoclonal, hybridoma
Ig Class IgG1
Immunogen Isolated from human seminal plasma
Specificity/Clinical Application The presence of prostate specific antigen is highly specific for demonstration of the prostatic origin of a malignancy. Mab 222MPA has been shown to be a valuable reagent for the identifica-tion of primary and metastatic prostatic carci-noma. The antibody reacts with a 34 kDa protein in benign and malignant prostatic epithelium.
Positive Control Prostatic benign hyperplasia
Application · Immunoblotting · Immunohistochemistry · Cell preparations
· suitable for frozen and paraffin sections*
* no pretreatment required
Antigen Recognized in Species
(tested so far) Human
Working Dilution 1:10
Dilution Buffer PBS with BSA, optimal dilution should be tested by serial dilution
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 1 ml
Gallee MPW et al.: Variation of prostate specific antigen expression in different tumor growth patterns present in prostatectomy specimens.
Urological Research 18, 181-187 (1990).
For In Vitro Research Use Only-Not For Use in or On Humans or Animals-Not for use in Diagnsotics
DATA SHEET :Mouse anti-Human Prostate Specific Antigen (PSA) (frozen & Paraffin Sections)
cat# RDI-PSAabm-A4 $375.00/vial
Preparation: Lyophilized antibody purified by Protein G affinity chromatography diluted in 1% w/v BSA in PBS with 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.
Clone: PSA 28/A4
Antigen: seminal fluid preparation
Specificity: Human prostate specific antigen (this antibody reacts with prostatic secretory and ductal epithelium in both normal and neoplastic tissue and can be useful in the identification of metastitic carninomas of prostatic origin)
USE: Effective on paraffin wax embedded tissue and on frozen sections.
Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 60 Minutes incubation at 25'C. Standard ABC technique.
Positive controls: normal prostate gland or known prostate carcinoma
Staining pattern: cytoplasmic
Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily. Stricly for in vitro research use only-Not for use in or on humans oranimals-not for use in diagnostics
Mouse anti-Prostate Specific Antigen (Cocktail) for WB & histo
cat# RDI-PSA85abm $375.00/100ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Immunogen: purified human PSA
Isotype: mIgG1 (both)
Clones: ER-PR8 & PA05
Reactivity: human(others not tested)
Uses: -Western blot: 1ug/ml 2 hr rt -Western blot: 1ug/ml 2 hr rt (recommend clone ER-PR8
alone for best westernblot reactivity cat#RDI-PSAR8abm $438.00/200ug
-histochemistry (frozen and paraffin sections) approx 1-2ug/ml 30-60 minutes RT
-for formalin fixed sections, requires pretreatment in boiling 10mM citrate bufer pH6.0 for 10-20 minutes followed by cooling at RT for 20 min.
Positive Contrl: prostate or prostate carcinoma
Background: Recognizes a single protein of 33-34kDa, identified as PSA. This "cocktail" is composed of two clones whose epitopes are not precisely mapped, but experimental data suggets that they are different. This cocktail is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate pithelium, and abundant in seninal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver derived serine protease inhibitor, alpha-1- antichymotrypsin. A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.
Storage: 2-8 deg c