CD29 Antibodies

CD29 Antibodies

Mouse anti-human CD29 antibodies

- 3clones  

CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CBL481                 TDM29                           --           mIgG1       purified     $375.00

RDI-CBL481FT                " "                                 "              "             FITC        $375.00

RDI-CBL481PE               " "                                  "              "             PE            $438.00

RDI-CBL481BT               " "                                  "              "            Biotin         $375.00

RDI-CBL481PC5             ""                                    "              "           RPE/CY5   $469.00

RDI-M2129clb                2A4                               --              "             purified     $438.00

RDI-M2233clb                 " "                                 " --           "             FITC        $438.00

RDI-CD29abm-P4          P4C10                            --             mIgG1    purified     $500.00

RDI-CD29abm-4B         4B7R                             --              mIgG1    purified     $312.00

RDI-CD29abm-4BPE    4B7R                             --              mIgG1    PE             $500.00/120T

RDI-CD29abm-4BFT    4B7R                             --              mIgG1    FITC         $375.00/120T

RDI-CD29abm-4BBT   4B7R                             --              mIgG1    Biotin        $375.00/100ug

Product Specification: mouse monoclonal CD29

Code: CLB-CD29

CAT#: RDI-M2129clb   $438.00/vial

Lot Number:

Tests/vial: 200

Specificity:

Cell type: Broad leucocytes

Ag.MW.: 130 kD.

Reactivity: The monoclonal antibody is directed against the CD29-antigen (gplla, VLA B-chain,integrin B1- chain),which can form distinct complexes with either of six different a-chains of the Very Late Antigens (VLA). The monoclonal antibody reacts with thrombocytes, monocytes and a subpopulation of T and B lymfocytes. The monoclonal antibody does not react with erythrocytes and weak with granulocytes.

Application: Functional studies on cells.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: 2A4

This clone has been derived from hybridization of X63 Ag 865-3 cells with spleen cells of BALB/c mouse immunized with U937 cells.

Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Mouse ascites fluid.

Purification: Protein G affinity chromatography.

Packing: Each vial contains 1 ml with approximately 200ug/ml monoclonal antibody and 1mg BSA in PBS.

Preservative: Sodium azide (0.1%).

Storage: Monoclonal antibodies should be stored at 2-8 Deg.C. Do not use this product beyond the stated expiration date.

Reference: 1.Humpries, M.J.,Cell Sci., 97,585-593 (1990).

FOR IN VITRO RESEARCH USE ONLY

Product Specificity: mouse monoclonal CD29,conjugated to   Fluorescein

Code: CLB-CD29-F

CAT#: RDI-M2233clb

Lot Number:

Tests/vial: 100

Specificity:

Cell type: Broad leucocytes

Ag.MW.: 130 kD.

Reactivity: The monoclonal antibody is directed against the CD29-antigen (gplla, VLA B-chain,integrin B1- chain),which can form distinct complexes with either of six different a-chains of the Very Late Antigens (VLA). The monoclonal antibody reacts with thrombocytes, monocytes and a subpopulation of T and B lymphcytes. The monoclonal antibody does not react with erythrocytes and weak with granulocytes.

Application: Functional studies on cells. Direct immunofluorescence staining with analysis by FACS.

purification: Protein G affinity chromatography.

Chemical Characteristics:

Molecular F/P ratio : 4.6

Packing: Each vial contains 1 ml conjugated monoclonal anti- body and 1 mg BSA in PBS.

Preservative: Sodium azide (0.1%).

Storage: Fluorescein labelled monoclonal antibodies should be stored at 2-8Deg.C and will retain their activity for approximately 12 months.

Reference: 1. Humpries, M.J.,Cell Sci., 97,585-592 (1990).

FOR IN VITRO RESEARCH USE ONLY

MOUSE MONOCLONAL ANTIBODY TO: HUMAN CD29

PRODUCT CODE   RDI-CBL481   $375.00/vial

CLONE    TDM29

ISOTYPE     IgG1

IMMUNIZING ANTIGEN   U937 cells

FUSION PARTNER   P3x63 Ag 8.653 myeloma cell line

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY    The antibody reacts with the integrin b1 chain which is a membrane glycoprotein (130 kDa) that can complex with the 6 different a-chains of the VLA's. It is also known as VLA b1-chain and the fibronectin receptor. The antigen is not expressed on erythrocytes and only weakly stains granulocytes.

Antigen distribution:

T cells             40 + 10%

CD4+ cells      40 + 20%

CD8+ cells      40 + 15%

B cells             20 + 5%

APPLICATIONS

*Immunoprecipitation studies of CD29 antigen

*Indirect immunofluorescence and FACS analysis for CD29 expressing cells

*Cell-cell and cell-matrix adhesion studies involving the CD29/CD49 heterodimers

REFERENCES

Hemler, M.E. Ann. Rev. Immunol. 8, 365-400 (1990)

Leucocyte Typing VI, Oxford University Press (in press)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: FITC labeled cat#RDI-CBL481FT $375.00/100T vial

PE labeled cat#RDI-CBL481PE            $469.00/100T

Biotin labeled cat#RDI-CBL481BT      $375.00/100T

Bulk (purified, NO BSA) ord cat#RDI-CBL481-1X $1062.00/1mg

No BSA, NO AZIDE      cat#RDI-CBL481-1XP $1062.00/1mg

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

MOUSE ANTI-HUMAN INTEGRIN b1 (cd29)   MONOCLONAL ANTIBODY

CATALOG# RDI-CD29abm-P4    $500.00

Presentation: 100ug in 0.1ml (1mg/ml)

CLONE: P4C10

ISOTYPE: IgG1

SPECIFICITY: Reacts with the B1 subunit of the integrin protein family. Appears to be human specific.

APPLICATIONS: -Inhibits attachment of Mg63 cells to collagen type I at a dilution of 1:1500. Inhibits endodermal and keratinocytes to collagen,fibronectin and laminin. It also inhibits cell to cell adhesion.

-Immunoflourescence

-Immunoblotting

-Immunoprecipitation

-ELISA

Optimal working dilutions must be determined by end user.

STORAGE: Maintain at 2-8C in undiluted aliquots for up to 6 months.

REFERENCES: 1. Carter, W. A. et al. 1990. J. Cell Biol. 110:1387

                          2. Wayner, E.A. et al. 1991. J. Cell Biol. 113: 919.

For research use only; not for use as a diagnostic.

also available as ascites cat#RDI-CD29abm-P4AS $438.00/0.1ml

Monoclonal anti-human CD29 (b1 integrin)/R-PE Conjugated clone 4B7R

cat# RDI-CD29abm-4BPE   $500.00/120T

Presentation: 120Tests in 0.5ml 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Clone: 4B7R

Isotype: Mouse IgG1k

Immunogen: Human ocular melanoma cell line, V+B2

PRODUCTION: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.8 mg/ml with an A565/A280 ratio of 1.58.

DILUTION: 1:20 (80ul/test)

INFORMATION: Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. CD29 can form heterodimer pairs with at least nine different alpha subunits. Antibody 4B7R recognizes the CD29 integrin subunit.

Activity: direct Flow cytometry

-in non-conjugated forms-Immunoprecipitation Frozen Sections

Fixed Formaldehyde Paraffin Sections

References: Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1612-1613.

STORAGE: Store at 2 - 5oC . Do not freeze! Protect from light.

PERFORMANCE: 500K cultured Nalm-6 cells were incubated 45 minutes on ice with 80 ul of anti-CD29/R-PE at a 1:20 dilution indicated above. Cells were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control (Cat#RDi- MIIG1PEX2 $188.00) at a similar concentration. Binding was 93% blocked when pre incubated 10 minutes with 20 ull of 0.5 mg/ml anti-CD29 antibody (Cat#RDI-CD29abm-4B)

Also available: intact

non conjugated cat#RDI-CD29abm-4B $312.00/100ug

FITC conjugated: cat#RDI-CD29abm-4BFT $375.00/120T

Biotin conjugated: cat#RDI-CD29abm-4BBT $375.00/100ug

PE conjugated: cat#RDI-CD29abm-4BPE $500.00/100T

-Phycoerythrin (R-PE) is covered under patents: U.S. 4,520,110; European 76,695 and Canadian 1,179,942.