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Peripherin Monoclonal Antibody
Catalog Number: RDI-ALS24508 $344.00/100ul
Description: Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
Host Species: Mouse
Specificity: 8G2 was raised against recombinant rat peripherin purified from E. coli. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of rat brain. 8G2 was one of several clones which stained processes expected to be peripherin positive. The antibody is clean and specific for the expected 57kDa band on Western blots. While subsequent studies indicated that 8G2 is relatively sensitive to aldehyde fixation and so is not recommended for heavily fixed formalin fixed sections of brain. 8G2 stains peripherin in a variety of mammalian species, but gives equivocal results on chicken tissues.
Applications: For immunofluorescence, use 8G2 diluted 1:500. For western blots use 8G2 diluted 1:5,000.
Format: Sterile cell culture fluid plus sodium azide. 8G2 is a mouse IgG1 with a kappa light chain. Supplied at a concentration of 1mg/ml.
Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.
References: Zhang X, Klueber KM, Guo Z, Lu C, Roisen FJ. Adult human olfactory neural progenitors cultured in defined medium. Exp Neurol. 186:112-23 (2004).
Fasani F, Bocquet A, Robert P, Peterson A, Eyer J. The amount of neurofilaments aggregated in the cell body is controlled by their increased sensitivity to trypsin-like proteases. J Cell Sci. 2004 Feb 3;Pt. [Epub ahead of print]
He and Hays. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54 (2004).
Larivière, R. C., Nguyen, M. D., Ribeiro-da-Silva, A. and Julien, J.-P. Reduced number of unmyelinated sensory axons in peripherin null mice. J. Neurochem. 81:525-532 (2002).
Chien, C. L., Lee, T. H and Lu, K, S. Distribution of neuronal intermediate filament proteins in the developing mouse olfactory system. J. Neurosci. Res. 54:353-63 (1998).
Errante LD, Wiche G and Shaw G. Distribution of plectin, an intermediate filament-associated protein, in the adult rat central nervous system J. Neurosci. Res. 37:515-528 (1994).
For research use only; not for use as a diagnostic
cat# RDI-PERIPHabm $500.00/vial
Background: Peripherin is a 57kD type III intermediate filament that is specific marker for peripheral neurons, including enteric ganglion cells. peripherin is expressed in the developing peripheral nervous system and is highly enriched in neuronal derivatives of the neural crest. the detection or absence of peripherin may be used to demonstrate abnormalities of the enteric nervous system. The histological diagnosis of Hirschsprung's disease (HD) is based on the absence of ganglion cells in the myenteric or submucosal nerve plexus of the affected bowel. The assessment of the density of ganglion cells is highly effective where peripherin is utilized as the marker and provides essential information for the diagnosis of HD disorders. Peripherin is utilized over other neural markers, such as S100 and NSE in that it does not stain chromaffin cell types. Peripherin is also expressed in neural crest derived tumors such as neuroblastomas and ganglioneuroblastomas making it a useful marker for these neoplasms.
Specificity:* recognizes human peripherin
Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.
Antigen: Prokaryotic recombinant fusion protein corresponding to the full length peripherin molecule
Hybridoma: mouse myeloma (p3-NS1-Ag4-1)
USES: -acetone fixed frozen sections
-Paraffin sections (using high temp unmasking technique, 0.01M citrate buffer pH 6.0, approx 10 minutes)
-typical dilutions:1:100-1:200, 60 minutes incubation at 25 DEG C with ABC detection
Controls: small bowel and neuroblastoma, peripheral nerves and ganglion cells
Reconstitution: 1 ml distilled water. Allow to set 30-60 minutes, mix well.
Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles. Store in non frost free freezer.
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