CYTOCHROME P450 RESEARCH PRODUCTS
CYTOCHROME P450 RESEARCH PRODUCTS
available through Research Diagnostics Inc
-See our NATIVE ISOLATES of the following-please click for more details: CYP2A6, CYP2C9, P450 reductase & cytochrome b5. Certain research has indicated that there may be some discrepancies between recombinant and native isolates. These native enzymes will allow you an opportunity to check your system for both types. A select group of recombinant enzymes are also now available :
-NEW:see also related GST (Glutathione Transferases) enzymes for drug metabolism research
Anti-Cytochrome P450 Antibodies
(rabbit polyclonal). P450 antibodies give 90% inhibition of prototypic reactions catalyzed by the corresponding Ag in intact liver microsomes. "All P450 abs except the CYP2C IgGs react specifically with their respective antigens on western blots. CYP2C9 and CYP2C19 abs cross-react with other CYP2C proteins in human liver (unless marked as specific). Available as purified rabbit IgG fractions (lyophilized).
Click on catalog number to view a sample spec sheet . Click here for a list of some references:
CATALOG# Price
RDI-CYP2A6abr $350.00/1mg
RDI-CYP2C8abr currently sold out
RDI-CYP2C9abr $350.00/mg
RDI-CYP2E1abr $350.00/1mg
RDI-CYP2C19Mabr $750.00/1mg (specially backabsorbed to reduce reactivity with CYP2C8 and CYP2C9)
RDI-CYP2C19abr $550.00/1mg (more sensitive for CYP2C19 but also sees CYP2C8 and CYP2C9
in full size blots
RDI-CYP3A4abr $350.00/mg (also sees 3A5 & 3A7)
RDI-CYP3A4Mabr $450.00/mg (specific for 3A4)
RDI-CYP2D6abrX $350.00/vial (at least 10 western blots)
RDI-CYP4A11abr $350.00/mg
RDI-CYP4F2abr $375.00/mg
RDI-CYP2C10abrX $325.00/vial (at least 10 western blots)
RDI-NORMCTRL (non immune control serum $100.00/mg)
additional antibodies of interest:
RDI-P450REDabr $325.00/0.1ml anti-cytochrome P450 reductase
RDI-P450SCCabr $325.00/0.1ml anti-cytochrome P450 side chain cleavage enzyme
RDI-CYP1A1abr $325.00/0.1ml anti-cytochrome P450 1A1
RDI-CYP1A2abr $325.00/0.1ml anti-cytochrome P450 1A2
RDI-CYP2B12abr $325.00/0.1ml anti-RAT cytochrome P450 2B1 & 2B2
RDI-RTCYP2C11abm $350.00/vial mouse anti-rat CYP450 2C11
RDI-RTCYP2C11abr $325.00/0.1ml anti-rat CYP2C11
RDI-CYP2A6B6abr $325.00/0.1ml anti-cytochrome P450 2B6 & 2A6
-also available(anti-human CYP1A1, CYP3A2, & CYP3A5)
-see also monoclonals to P450's
see also antibodies to rat: CYP1A1, CYP1A2, CYP2B1/2, CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A2, CYP4A1/2/3, CYP450 reductase, CYP 450 side chain cleavage and other against human
-please inquire for antibodies against other CYP enzymes.
CYTOCHROME P450 Research Products
Suggested Protocol for Standard Reconstitution recombinant Cytochrome P450's:
-ref: Dieter, H. and Johnson, E.F. (1982) J. Biol. Chem. 257:9315-9323.
Components: Cytochrome P450
Cytochrome P450 Reductase
Lipid (L-alpha-phosphatidylcholine dilauroyl ( C 12:0) Sigma Chemical cat#P-1263
NADPH
50mM Potassium Phosphate Buffer pH 7.4 (KPi buffer) pH by mixing monobasic and dibasic KPi
Preparation of lipid: A stock solution (3mg/ml) is prepared fresh in KPi buffer. Prior to use, the lipid solution must be dispersed by four 5 second pulses at 20% power using a Branson 200 series sonifier (or similar device) fitted with a microprobe.
Reconstitution: To assess substrate metabolism, combine 10 pmole cytochrome P450 (example: 10ul of a 1 nmole/ml solution) with 0.3 units cytochrome P450 reductase (example 10ul of a 30 unit/ml solution) and 30ug freshly prepared lipid (example 10ul of a 3 mg/ml solution) and incubate on ice for 10 minutes. To this add the substrate and kPi buffer to a final volume of 980ul and mix (in most cases, the final concentration of the substrate solvent should not exceed 1%). Incubate this mixture for 3 minutes at 37 DEG C. The reaction is initiated by the addition of NADPH to a final concentration of 1mM (20ul of a 50 mM stock solution).
Reductase Dilution buffer: 10mM KPi, pH 7.4, 20% glycerol, 0.1Mm EDTA.
Cytochrome P450's and select substrates
Cytochrome P450 2C8: taxol, retinoic acid, benzo{a}pyrene, arachidonic acid, warfarin
Cytochrome P450 2C9: tolbutamide, phenytoin, arachidonic acid, intienilic acid, warfarin
Cytochrome P450 2C18: warfarin
Cytochrome P450 2C19: S-mephenytoin, diazepam, tolbutamide, progesterone, warfarin
Cytochrome P450 2D6: debrisoquine, sparteine, bufuraol, warfarin
CYTOCHROME P450 INHIBITORS
Below are some of the various compounds with known inhibition activity of the cytochrome P450 enzyme. Please contact us for additional compounds. All materials are for in vitro research use only-not for use in diagnostics-not for use in or on humans or animals .
CAT# Description Price
RDI-R41400 R 41400 A.S. Ketoconazole 99+% 50mg $250.00
(inhibits Cp450c17) Kuhn-Velten et al, Biochem. Pharmacol. 44 (12), 2371 (1992).
RDI-R51211 R 51211 A.S. Itraconazole 99+% 50mg $250.00
RDI-R18134 R 18134 A.S. Miconazole 99+% 50mg $250.00
RDI-R14950 R 14950 A.S. Miconazole Nitrate 99+% 50mg $250.00