CD147 Antibodies

CD147 Antibodies

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Mouse anti-human CD147  antibodies (Neurothelin)

CATALOG#                 CLONE#                  Workshop    Host         Form            Price

RDI-CBL535                   Mem-M6/1                  VI            mIgG1      purified       375.00/200ug

RDI-CBL535FT              Mem-M6/1                  VI              "               FITC           375.00/100T

RDI-CBL572                   CB43                          VI            mIgG1         purified       375.00/200ug

RDI-CBL572FT                    "                             "                "               FITC            375.00/100T

RDI-CBL572PE                   "                              "                "               PE                94.00/100T

RDI-CD147-M6              HIM6                          VI            mIgG1         purified        4062.00/100ug

RDI-CD147-D6               UM-8D6                     VI            mIgG1         purified        €112.00/100ug

 

 

DATA SHEET: Mouse anti-human CD147

Catalog#: RDI-CD147-M6    $312.00/vial    

-also available: Fitc labeled cat#RDI-CD147-M6FT   $438.00/100tests

 

Package Size: 100ug purified in 0.3ml (0.5mg/ml) 50mM Tris-Hcl   (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits- avoid contact with acids.)

Prep: purified

Species: mouse IgG1k

CLONE: HIM6 Workshop Code:VI, N-L109

Activity: Reacts with basigin or neurothelin, a transmembrane glycoprotein (50-60kd) of the immunoglobulin super-gene family. Neurothelin is a blood brain barrier-specific molecule. It bears homology with mouse gp42 or basigin, human M6 or EmMprin, rat OX-47 or CE-9, and avian HT7 or 5A11. Neurothelin is a molecule which is broadly expressed on cells of hematopoietic and non-hematopoietic origin. Its expression on specific cell types may be regulated by cytokines. CD147 plays a role in embryonal blood-brain barrier development and a role in integrin-mediated adhesion in brain endothelia. Binds to epitope 1 of CD147.

 

Application: -Indirect immunofluorescence cell surface staining (flow cytometry) , Titer for each application

(does not inhibit homotypic aaggregation or adhesion).

-histochemistry (showing cytoplasmic and membrane positivity in anaplastic elements of squamous carcinoma of the lung) tested on paraffin sections using Streptavidin:Biotin detection.

-immunoprecipitation (48kda from HPB-ALL cells)

 

Storage: Store at 4 DEG C.

 

Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or   for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-human CD147 clone CB43

cat#RDI-CBL572            $375.00/200ug

cat#RDI-CBL572FT        $375.00/100t FITC conjugated

cat#RDI-CBL572PE        $469.00/100T PE conjugated

 

CLONE                                    CB-43

ISOTYPE                                 IgM

SOURCE                                  Tissue culture supernatent

PURIFICATION METHOD     Protein A affinity chromatography

SPECIFICITY The antibody recognizes the 50-60kDa CD147 antigen that is broadly expressed on cells of both haematopoietic and non-haematopoietic derivation. The antigen is upregulated upon cell activation in particular on lymphocytes and monocytes.

APPLICATIONS * Flow cytometry* Studies on tumour cells where CD147 is believed to bind a fibroblast ligand which stimulates the production of collagenase and other extracellular matrix metalloproteinases.

 


Mouse anti-Human CD147


PRODUCT CODE RDI-CBL535      $375.00/vial 200ug


CLONE: Mem -M6/1

ISOTYPE: IgG1

SOURCE: mouse ascities

PURIFICATION ion exchange chromatography

SPECIFICITY: This antibody recognizes 50-60Kda cell surface protein which is the human homologue of rat OX-47 antigen. (This moiety is expressed in blood brain barrier endothelium, on normal and neoplastic breast tissue)


APPLICATIONS: *Flow Cytometric studies of the expression of CD147 (also known as neurothelin)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in in phosphate buffred saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

 

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

 

Bulk, milligram (NO BSA, no AZIDE) cat#RDI-CBL535-1XP $1062.00/mg


mouse anti-human CD147 clone UM-8D6, mIgG1k

cat#RDI-CD147-D6             $312.00/100ug purified

cat#RDI-CD147-D6PE         $500.00/120T PE conjugated

cat#RDI-CD147-D6FT         $475.00/120T FITC conjugated

cat#RDI-CD147-D6BT         $475.00/100ug Biotin conj

 

-clone SUM-8D6 recognizes the CD147 molecule of about 35-40kd.In its purfied format it will inhibit homotypic aggregation, adhesion to matrix proteins and migration through matrigel

-suitable for flow cytometry, immunoprecipitation, acetone fixed frozen sections

-immunogen:Human T cell line MOLT 13

-ref: -Leukecyte Typing VI (T. Kishimoto et al, eds) Garland Publishing Inc, New York (1997) p760-766

-A.H. Kirsch et al (1997) Tissue Antigens 50:147-152


Mouse anti-human CD147 (Neurothelin/Basigin):PE conjugated clone UM-8D      

cat#RDI-CD147-D6       $312.00/100ug purified

cat#RDI-CD147-D6PE $500.00/120T PE conjugated

cat#RDI-CD147-D6FT $475.00/120T FITC conjugated

cat#RDI-CD147-D6BT $475.00/100ug Biotin conj

 

Package Size: 120Tests in 0.2ml 50mM sodium phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% glycerol, 0.2% BSA & 0.04% NaN3.

 

Prep: Protein A purified antibody from TCS conjugated to PE. Unconjugated PE removed by size exclusion column. (approx 0.5mg/ml) A565/A280 3.4

 

Species: mouse IgG1k

 

Immunogen: Human T cell line MOLT 13

 

CLONE: UM-8D6

 

Activity: -clone UM-8D6 recognizes the CD147 molecule of about 35-40kd.In its purfied format it will inhibit homotypic aggregation, adhesion to matrix proteins and migration through matrigel

-suitable for flow cytometry, immunoprecipitation, acetone fixed frozen sections

 

Application: 500K cultured Raji cells per tube were washed and incubated 45 minutes on ice with 80ul of the anti- CD147:PE antibody diluted 1:50 at a concentration of 10ug/ml. Cells were washed three times, fixed and analyzed using a BD FACSCalibur. Cells stained positive with a mean shift of 2.26 log 10 fluorescent units when compared to a mouse IgG1:PE

(cat#RDI-MIGG1X2PE $312.00/100ug negative control at a similar concentration.

 

Storage: Store at 4 DEG C. Protect from Light-DO Not Freeze

 

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.