Cytokeratin 3 (clone AE5) antibodies from RDI Divison
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 3
MOUSE MONOCLONAL ANTIBODY TO: HUMAN CYTOKERATIN 3
Mab to Cytokeratin 3/12
Cat#RDI-PRO61807 (same as RDI-CBL218) $440.00
Clone Determination AE 5
Category Mouse monoclonal
Immunoglobulin Class IgG 1
Purification Ion-exchange chromatography
Antigen Human epithelial keratin
Specificity AE 5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells.
Application Suitable for immunohistochemistry of frozen tissue Immunoblotting (Western)
Polypeptide Reacting MR 64 000 polypeptide (cytokeratin 3) of human corneal
epithelium and cytokeratin 2 "p" of palate epithelium
Antigen Recognized in Human, rabbit, bovine (in cow and
Species (tested so far) rabbit AE5 reacts with lip and snout epithelia)
Reactivities on Cultured Rabbit corneal epithelial cells
Cell Lines (tested so far)
Tissues Specifically Detected Cornea and palate epithelium
Application Suitable for frozen tissue Immunoblotting (Western)
Working Dilution Dilute 1:50 with PBS, pH 7.2 for immunohistochemical
application and 1:500 for immunoblotting
Incubation Time 1h at RT
Storage At 2-8°C; for longer storage we recommend storage in
aliquots at or below -20°C
Quantity 200 µg [1mg/ml] in PBS (pH 7.2)
References
Cooper, D., Schermer, A. and Sun, T.-T.: Classification of human epithelia and their neoplasms using monoclonal antikeratin antibodies: Strategies, applications and limitations. Lab. Invest. 52, 243-256 (1985).
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Schermer, A., Galvin, S. and Sun, T.-T.: Differentiation-related expression of a major 64 K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J. Cell Biol. 103, 49-62 (1986).
Sun, T.-T., Eichner, R., Cooper, D., Schermer, A., Nelson, W.G. and Weiss, R.A.: Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. The Cancer Cell 1, 169-176 (1984
Cat. No. RDI-PRO61807
For research use only. Not supplied for use in human diagnostic or therapeutic procedures.