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Mouse anti-human CD32 antibodies
-3 clones clones CIKM5 and IV3 = primarily FCGRIIa reactive ,
clone FLI8.26 reactive with both FCGRIIa & IIb , clone 7.3 domain 2 of all epitopes
CATALOG# CLONE# Workshop Host Form Price
RDI-M2202clb CIKM5 IV mIgG1 purified $438.00
RDI-CD32-FLI8 FLI8.26 V mIgG2b purified $281.00
RDI-CD32-FL18FT " " " FITC $438.00
RDI-CD32abm-73 7.3 -- mIgG1 purified 250.00/100ug
RDI-CD32abm-7BT 7.3 -- mIgG1 Biotin $375.00/100ug
RDI-CD32abm-7PE 7.3 -- mIgG1 PE $500.00/100T
RDI-CD32abm-7X2 7.3 -- mIgG1 F(ab')2 $1000.00
RDI-CD32abm-FAb 7.3 -- mIgG1 FAB $1000.00/100ug
Product Specification: mouse monoclonal anti-human CD32
CAT#: RDI-M2202clb $438.00/vial
Cell type: Macrophages,granulocytes,B-cells,eosinophils
Ag.MW.: 39-48 kD.
Reactivity: The monoclonal antibody is directed against the the CD32-antigen,which is expressed on granulocytes,B-cells, eosinophils and macrophages (1,2). The CD32-antigen is the FcR-II receptor for aggregated IgG, and it has also a function in cell activation.The presence of CD32-antigen has prognostic significance in patients presenting with AML.Acute leukaemias with a high proportion of cell bearing the CD32-antigen may be similar in prognosis to the chronis forms of leukaemia (3,4).
Application: Diagnosis of acute leukaemias by flow cytometry.
Immunoperoxidase staining of frozen tissue sections
Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.
Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.
Source: Mouse ascited fluid.
Packing: Each vial contains 1 ml with approximately 200ug/ml monoclonal antibody and 1 ml BSA in PBS.
Preservative: Sodium azide (0.1%).
Storage: Monoclonal antibodies should be stored at 2-8Deg.C and will retain their activity for approximately 12 months.
Reference: 1. Tetteroo,P.A.T.et al.,McMichael,A.et atl.(editor)
Three differenct types of Fcy receptors on human
leucocytes defined by workshop antibodies: FcyR
low) of neutrophils,FcyR(low) of K/NK lymphocytes
and FcyRII, In Leucocyte Typing III,Oxford University Press, 702 (1987).
2. Pilkington,G.R.et al.,Knapp,W.(editor),Correlation of CD surface antigen phenotype with FAB subtype,remission induction (CR),and survival in AML patients,Leucocyte typing IV,Oxford University Press, 931 (1989).
3. Micklem,K.J.et al.,Different isoforms of human FcRII distinguished by CD32 antibodies,J.Immunol. 144,22295-2303 (1990).
4. Tucker, J.et al.,Immunophenotype of blast cells in acute myeloid leukaemia may be a useful predictive factor for outcome,Haematological Oncology, 8,47-58 (1990).
FOR IN VITRO RESEARCH USE ONLY
DATA SHEET: mouse anti-human CDw32 clone FLI8.26
(FCGRIIa, IIb and IIb1)
Catalog#: RDI-CD32FLI8 $281.00/vial $250.00/vial 3 or more
also available: FITC labeled cat#RDI-CD32FLI8-FT $438.00/100 tests
Package Size: 100ug purified in 0.2ml (0.5mg/ml) 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)
Prep: purified from tissue culture supernatant via Protein G affinity chromatography.
Species: mouse IgG2b
Workshop Code: Workshop V, MA128
Activity: Reacts with the 40KD type I membrane glycoprotein, FCgammaRII ) , the low affinity receptor for aggregated IgG expressed on B cells, monocytes, granulocytes and platelets. CDw32 mediates endocytosis, cytotoxicity, platelet activation and immunomodulation. Studies indicate this clone binds with FCgammaIIa, IIb1 and IIb1*.
Application: -Indirect immunofluorescence cell surface staining , Titer for each application
1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY. pages 832-835.
2) EMBO J. 8:3657
3) Blood 80:2261
4) J. Immunol 150:1794
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Monoclonal anti-human CD32(Fc gamma RII) F(ab')2
cat# RDI-CD32abm-7X2 $1000.00/100ug Bulk on request
Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added.
Isotype: Mouse IgG1
Immunogen: Human K562 tumor cells and L cells transfected with human Fc gamma RII
PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Ficin. F(ab')2 was separated from intact antibody and Fc fragments by protein A and Size exclusion chromatography. Purity was >90% F(ab')2 by SDS-PAGE.
Activity: Antibody 7.3 reacts with a domain 2 epitope or all CD32 isoforms. Intact and Fab'2 7.3 antibody blocks immune complex binding.
STORAGE : Store at 2 - 5oC. Open under aseptic conditions. Freeze/Thawing is not recommended.
INFORMATION: Human CD32 exists in at least six isoforms originating from three different genes (FcgRII A, B and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses.
References: Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 823-836.
PERFORMANCE: Five x 10 5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD32 F(ab')2 at 20 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC , after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
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