RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-Adeno-Asscociated Virus , intact particles

see other AAV antibodies and AAV Elisa kit

Mab to Adeno-Associated Virus, intact particles

cat#RDI-PRO61055 $500.00/vial Bulk Quotes on Request

Clone Determination A20

Category Mouse monoclonal

Immunoglobulin Class IgG3

Purification affinity purified from tissue culture

Antigen: Adeno-associated virus capsid proteins and virus particles

Specificity: For characterization of different stages of infection and very useful for the analysis of AAV assembly process. Clone A20 reacts specifically with INTACT adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.. The antibody cannot be used for immunoblotting. This antibody is also useful for neutralizing experimentss (see Moskalenko et al)

Epitope Mapping experiments identified four immunoreactive (dsicontinuous) regions. The major reaction was attributed to sequence aa369 to aa378 of AAV-2 capsids.

Application - immunofluorescence, Immunohistochemistry & immunoprecipitation, affinity chromatography, ELISA, neutralization

Species reactivity: AAV-2, found in human, monkey, in Elisa also applicable to AAV-3

Working dilution: After reconstitution with 1ml distilled water, 1:20 in immunofluorescence, 1:5 in immunoprecipitation

Dilution Buffer: PBS or TRIS-buffered saline

Incubation Time" Overnight at 2-8 DEG C for use in immunohistochemistry or immunofluorescence microscopy

Stability at 2-8°C one year after reconstitution with 1ml H20. Dilute with PBS.

Quantity 50 µg (lyoph.) contains 0.09% sodium azide and 0.05% BSA in PBS.

ref:

Wobus C.A., Hügle-Dörr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293.

J. Virology 74, 9281-9293 (2000)

Moskalenko M et al, J Virology 74, 1761-1766 (2000)

Gene Ther 6(7) 1322-1330 (1999)

Wistuba A. Weger S., Kern A. and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly:Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.

Wistuba A. Weger S., Kern A. and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures.