Product Description
Vinculin polyclonal antibody is available at gentaur for next week delivery
Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail . In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration. Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction. This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth. This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Vinculin polyclonal antibody detects endogenous levels of Vinculin protein.
W4BiowMW: ~ 130 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human Vinculin.Alternative Name:
Vinculin; Metavinculin; MV; VCLWestern blot analysis: Western blot (WB) analysis of Vinculin polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HepG2 whole cell lysate LaneC:PC12 whole cell lysate LaneD:The Muscle tissue lysate of Mouse
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P18206
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