Product Description
Vimentin (Phospho-S39) polyclonal antibody is available at gentaur for next week delivery
Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Vimentin (Phospho-S39) polyclonal antibody detects endogenous levels of Vimentin protein only when phosphorylated at Ser39.
W4BiowMW: ~ 57 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human Vimentin around the phosphorylation site of Serine 39.Alternative Name:
Vimentin; VIMWestern blot analysis: Western blot (WB) analysis of Vimentin (Phospho-S39) polyclonal antibody at 1:500 dilution LaneA:PMVEC whole cell lysate LaneB:A2780 whole cell lysate LaneC:3T3-L1 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P08670
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