Product Description
Vimentin (L79) polyclonal antibody is available at gentaur for next week delivery
Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Vimentin (L79) polyclonal antibody detects endogenous levels of Vimentin protein.
W4BiowMW: ~ 57 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 31-80 of Human Vimentin.Alternative Name:
Vimentin; VIMWestern blot analysis: Western blot (WB) analysis of Vimentin (L79) pAb at 1:1000 dilution Lane1:The testis tissue lysate of Rat(40ug) Lane2:The kidney tissue lysate of Mouse(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug) Lane5:Jurkat whole cell lysate (40ug) Lane6:PC3 whole cell lysate (40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Vimentin (L79) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P08670
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