VCL polyclonal antibody

Product Description

VCL polyclonal antibody is available at gentaur for next week delivery

Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail . In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration. Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction. This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth. This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  VCL polyclonal antibody detects endogenous levels of VCL protein.

W4BiowMW:  ~ 130kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to Human VCL.

Alternative Name:

Vinculin;Metavinculin;MV;VCL

Western blot analysis:  Western blot (WB) analysis of VCL polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug) Lane7:Hela whole cell lysate(40ug)

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P18206

 

 

 

 

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