Product Description
UBE2U polyclonal antibody is available at gentaur for next week delivery
Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: UBE2U polyclonal antibody detects endogenous levels of UBE2U protein.
W4BiowMW: ~ 42 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human UBE2U.Alternative Name:
Ubiquitin-conjugating enzyme E2 U; E2 ubiquitin-conjugating enzyme U; Ubiquitin carrier protein U; Ubiquitin-protein ligase UWestern blot analysis: Western blot analysis of extracts of various cell lines, using UBE2U antibody.
Immunohistochemistry: Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody at dilution of 1:100 (40x lens).
Host: Rabbit
Swiss-Prot: Q5VVX9
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