RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mab to Glial Filament Protein
cat#RDI-PRO61011 $375.00/vial BULK ON REQUEST
Clone Determination GF 12.24
Category Mouse monoclonal
Immunoglobulin Class IgG2a
Purification SpAss-Protein A (recombinant)
Antigen Intermediate filament cytoskeleton from cultured human glioma cells
Specificity GF 12.24 represents an excellent marker for cell typing; prenatal diagnosis of neural tube defects
Application Suitable for frozen and paraffin- embedded tissue (P)
Cytological material
Immunoblotting (Western)
Species: Human, bovine, rat and mouse
Stability at 4°C one year after reconstitution
Quantity 50 µg (lyoph.) (with BSA and azide) Bulk on request
For In Vitro Research Use Only
Pab to Glial Fibrillary Acidic Protein
cat#RDI-PRO10555 $312.00/0/.25ml vial
Category Rabbit polyclonal
Purification/Form Rabbit serum
Immunogen Isolated from human spinal cord.
Clinical Application/ Specificity The antibody is directed against the 56 kDa GFAP protein, the main subunit of intermediate filaments of glial cells and astrocytes. The antibody can be used to discriminate glial tumors (astrocytomas, ependymonas) from other tumors.
Application Immunohistochemistry on frozen and paraffin-embedded tissue sections.
(Proteolytic treatment not required)
Immunoblotting
Antigen Recognized in species(tested so far) Human, cow, rat
Recommended Positive Control Brain tissue
Working Dilution Dilute 1:50; optimal dilution should be tested with serial dilution.
Dilution Buffer 0.15 M Phosphate buffered saline with 1% BSA and 0.1% Na-Azide
Storage/Stability At 4°C for min. 1 year
Quantity 0.25 ml; 0.09% NaN3 as preservative
Reference:
Perentes, E. et al. Recent Applications of Immunoperoxidase Histochemistry in Human Neuro-Oncology (1987). Archiv Path. Lab. Med. 111:796-812
Mouse anti-GFAP (moncolonal cocktail)
-cat#RDI-GFAPabm-CK $562.00/0.5mg
-clones 4A11, 1B4 and 2E1 (equal protions)
-all mouse IgG2b
-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken
-use: western blot, indirect immunofluorescence of tissue cultured cells
and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue ections
-immunogen: bovine spinal cord homogenate
-note: by use of a cocktail, gerater overall sensitivity may be seen in histochemistry
-ref: Neurochem Pathol. 3:119-138 (1985)
-ref: J Neuropath Exp Neuro 45:692-703 (1986)
Mouse anti-GFAP (clone 4A11)
-cat#RDI-GFAPabm-411 $469.00/0.5mg
-clones 4A11
-mouse IgG2b
-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken
-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections
-immunogen: bovine spinal cord homogenate
-ref: Neurochem Pathol. 3:119-138 (1985)
-ref: J Neuropath Exp Neuro 45:692-703 (1986)
Mouse anti-GFAP (clone 1B4)
-cat#RDI-GFAPabm-1B4 $469.00/0.5mg
-clones 1B4
-mouse IgG2b
-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken
-use: western blot, indirect immunofluorescence of tissue cultured cells and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections
-immunogen: bovine spinal cord homogenate
-ref: Neurochem Pathol. 3:119-138 (1985)
-ref: J Neuropath Exp Neuro 45:692-703 (1986)
Mouse anti-GFAP (clone 2E1
-cat#RDI-GFAPabm-2E1 $469.00/0.5mg
-clones 2E1
-mouse IgG2b
-specific for GFAP. Has broad reactivity recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken
-use: western blot, indirect immunofluorescence of tissue cultured cells
and immuohistochemical staining of frozen sections, and formalin-fixed paraffin embedded brain tissue sections
-immunogen: bovine spinal cord homogenate
-ref: Neurochem Pathol. 3:119-138 (1985)
-ref: J Neuropath Exp Neuro 45:692-703 (1986)