Product Description
TOP2A (phospho-S1106) polyclonal antibody is available at gentaur for next week delivery
Background: DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: TOP2A(phospho-S1106) polyclonal antibody detects endogenous levels of TOP2A protein only when phosphorylated at Ser1106 .
W4BiowMW: ~ 180 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human TOP2A around the phosphorylation site of Serine1106.Alternative Name:
DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme; TOP2A; TOP2Western blot analysis: Western blot (WB) analysis of TOP2A (phospho-S1106) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:HuT78 whole cell lysate(40ug) Lane3:The Testis tissue lysate of Mouse(40ug) Lane4:The Testis tissue lysate of Rat(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P11388
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