Product Description
TK1 (phospho-Ser13) polyclonal antibody is available at gentaur for next week delivery
Background: Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer.
Applications: WB
Purification&Purity: The antibody was affinity-purified from Mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: TK1(Phospho-Ser13) polyclonal antibody detects endogenous levels of TK1 protein only when phosphorylated at Ser13.
W4BiowMW: ~ 26 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human TK1 around the phosphorylation site of Serine 13.Alternative Name:
Thymidine kinase, cytosolic; TK1Western blot analysis: Western blot (WB) analysis of TK1(Phospho-Ser13) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(20ug) Lane4:Jurkat whole cell lysate(30ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P04183
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