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  • TEF-1 polyclonal antibody
  • TEF-1 polyclonal antibody
  • TEF-1 polyclonal antibody

    TEF-1 polyclonal antibody

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    SKU:
    BS5961
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    Product Description

    TEF-1 polyclonal antibody is available at gentaur for next week delivery

    Background: A member of the TEA/ATTS domain family, Transcriptional enhancer factor 1 (TEF-1) is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain; TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

    Applications:  WB IHC ICC

    Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

    Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

    Specificity:  TEF-1 polyclonal antibody detects endogenous levels of TEF-1 protein.

    W4BiowMW:  ~ 48 kDa

    Reactivity: Human,Mouse,Rat

    Note:  For research use only, not for use in diagnostic procedure.

    Immunogen:

    Synthetic peptide, corresponding to amino acids 105-150 of Human TEF-1.

    Alternative Name:

    Transcriptional enhancer factor TEF-1; NTEF-1; NTEF1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13; TEAD1; TCF13; TEF1

    Western blot analysis:  Western blot analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 5% nonfat dry milk in TBST. Exposure time: 120s.

    Immunohistochemistry: Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence analysis: Immunofluorescence - TEAD1 Polyclonal Antibody Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    Host:  Rabbit

    Swiss-Prot: P28347

     

     

     

     

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