Product Description
Stat3 (phospho-S727) polyclonal antibody is available at gentaur for next week delivery
Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Applications: WB IHC IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-Stat3 (S727) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Ser727.
W4BiowMW: ~ 88 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Serine 727.Alternative Name:
Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRFWestern blot analysis: Western blot (WB) analysis of Stat3 (phospho-S727) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40µg) Lane2:A549 whole cell lysate(40µg) Lane3:L02 whole cell lysate(40µg) Lane4:PC12 whole cell lysate(40µg) Lane5:3T3-L1 whole cell lysate(40µg)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of p-Stat3 (S727) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis: Western blot (WB) analysis of Stat3 (phospho-S727) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 5 minutes whole cell lysate Lane3:HEK293T treated with TNFα(20ng/ml)for 20 minutes whole cell lysate Lane4:HEK293T treated with TNFα(20ng/ml)for 40 minutes whole cell lysate
Host: Rabbit
Swiss-Prot: P40763
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