SREBP-1 (phospho-S439) polyclonal antibody

Product Description

SREBP-1 (phospho-S439) polyclonal antibody is available at gentaur for next week delivery

Background: Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  SREBP-1 (phospho-S439) polyclonal antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at Ser439.

W4BiowMW:  ~ 121 kDa

Reactivity: Human,Rat,Mouse

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human SREBP-1 around the phosphorylation site of Serine 439

Alternative Name:

Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1; SREBF1; BHLHD1; SREBP1

Western blot analysis:  Western blot (WB) analysis of SREBP-1 (phospho-S439) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P36956

 

 

 

 

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