Product Description
SQSTM1 polyclonal antibody is available at gentaur for next week delivery
Background: Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. Protein aggregates formed by SQSTM1 can be degraded by the autophagosome. SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome. Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: SQSTM1 polyclonal antibody detects endogenous levels of SQSTM1 protein.
W4BiowMW: ~ 62kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human SQSTM1.Alternative Name:
Sequestosome-1;EBI3-associated protein of 60 kDa;EBIAP;p60;Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Ubiquitin-binding protein p62;SQSTM1Western blot analysis: Western blot (WB) analysis of SQSTM1 polyclonal antibody at 1:2000 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:THP-1 whole cell lysate(20ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:Raw264.7 whole cell lysate(40ug) Lane6:The pancreas tissue lysate of mouse(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q13501
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