Product Description
Skp1 p19 (P72) polyclonal antibody is available at gentaur for next week delivery
Background: Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Skp1 p19 (P72) polyclonal antibody detects endogenous levels of Skp1 p19 protein.
W4BiowMW: ~ 19 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 40-90 of Human Skp1 p19.Alternative Name:
p19A; RNA polymerase II elongation factor-like protein; Organ of Corti protein 2; OCP-2; OCP-II; Transcription elongation factor B; SIII; SKP1; EMC19; OCP2; SKP1A; TCEB1LWestern blot analysis: Western blot (WB) analysis of SKP1 pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Skp1 p19 (P72) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P63208
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