Product Description
SECISBP2 polyclonal antibody is available at gentaur for next week delivery
Background: Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3' untranslated region of the mRNA to decode the UGA codon as Sec. SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins. Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA. SBP-2 shows binding activity in the liver and testis as well as hepatoma cells. SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1. A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells. SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements. SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: SECISBP2 polyclonal antibody detects endogenous levels of SECISBP2 protein.
W4BiowMW: ~ 110 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human SECISBP2.Alternative Name:
Selenocysteine insertion sequence-binding protein 2; SECIS-binding protein 2; SECISBP2; SBP2Western blot analysis: Western blot analysis of extracts of various cells, using SECISBP2 antibody.
Immunohistochemistry: Immunohistochemistry of paraffin-embedded human liver injury using SECISBP2 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded human colon carcinoma using SECISBP2 antibody at dilution of 1:200 (40x lens).
Host: Rabbit
Swiss-Prot: Q96T21
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