Product Description
RNF144B polyclonal antibody is available at gentaur for next week delivery
Background: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: RNF144B polyclonal antibody detects endogenous levels of RNF144B protein.
W4BiowMW: ~ 34 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human RNF144B.Alternative Name:
E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein; RNF144B; IBRDC2; P53RFPWestern blot analysis: Western blot (WB) analysis of RNF144B polyclonal antibody at 1:500 dilution LaneA:H1792 whole cell lysate LaneB:A549 whole cell lysate LaneC:CT26 whole cell lysate LaneD:PC12 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q7Z419
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