RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD100 Antibodies
Mouse anti-human CD100 antibodies
-2clones
CATALOG# CLONE# Workshop Host Form Price
RDI-CD100-A8 A8 V mIgG1 purified $312.00
RDI-CD100abm-30 20 N/A mIgG1 purified $440.00
see also polyclonals
DATA SHEET: Mouse anti-human CD100 clone A8
Catalog#: RDI-CD100-A8 $312.00/vial $281.00/vial 3 or more
also available FITC labeled cat#RDI-CD100-A8FT $406.00/100 tests
Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)
Prep: purified from tissue culture supernatant via Protein G affinity chromatography.
Species: mouse IgG1,k
CLONE: A8
Workshop Code: Workshop V, BP383,S233
Activity: Reacts with human CD100, a 150kD homodimer cell surface antigen expressed on most hematopoietic cells, except immature bone marrow cells. CD100 is detectable on NK cells, EBV-transformed B cells, monocytes and tunmor T cell lines. It has been labeled a "semaphorin" because of its ability to modify CD40-CD154 B cell signalling by augmening B cell aggregation and survival.
Application: -Indirect immunofluorescence cell surface staining , Titer for each application
References: 1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY.
2) J. immunol 148:318
3) Proc Natld Acad Sci 93:11780
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Mouse anti-CD100 (Human/Mouse/Rat/Rabbit)
cat# RDI-CD100abm-30 $440.00/50ug
RDI-CD100abm-30X $625.00/150ug
CD100 was originally described as a 150kDa surface T-cell antigen induced after T-cell activation. Human CD100 exhibits widespread gene expression but is most prominent in peripheral lymphocytes, thymus, skeletal muscle, and brain. The full CD100 protein is 862 amino acids with homolgy to the semaphorin family of proteins at its extracellular domain followed by a short Ig-like domain. The short cytoplasmic domain contains a putative tyrosine phosphorylation site which may be the site for association with intracellular kinases. Cells that overexpress CD100 induce a rapid aggregation of B cells and increase the CD90L-B cell aggregation. Because semaphorins are axon-guiding molecules for the nervous system, it has been proposed that CD100 may have a guidance role in the immune system.
Antigen: derived from human CD100 aa 721-861 (cytoplasmic) sequence
isotype: mIgG1
Clone ref: 30
package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.
Use: Suitable for western blot on human, rat and mouse using approx 0.25ug/ml ab on total cell lysate. Major band at Mw 150kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection. Also tested for immunofluorescnece and histochemsitry (positive control rabbit spleen)
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.
Main REF: Hal, KT et al, 1996 Proc Natl Acad Sci USA 93:1178-- 11785
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
-copyright by owner
Anti-CD100 Polyclonal Antibody ( C term)
Catalog Number: RDI-ALS24202 $312.00/vial $250.00/vial 5+
Quantity: 100ug in 0.1ml (1mg/ml) PBS containing 0.02% sodium azide.
Bulk: cat#RDI-ALS24202X1 $1875.00/1mg
Description: Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B-cell signaling by augmenting B-cell aggregation and survival and down-regulating CD23 expression. CD72 is a lymphocyte receptor for the Class IV semaphorin CD100, which is involved in regulating B cell signaling.
Host Species: Rabbit
Specificity: Reacts with residues 777-788 [KPKSDFCDREQS] of the human CD100 protein.
Applications: Western blot - Use at 1:500 to 1:1,000 dilution.
Format: Purified IgG
Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.
Reference: Hall et al. (1996).Proc Natl Acad Sci U S A 93(21):11780-5
For research use only; not for use as a diagnostic.
Anti-CD100 Polyclonal Antibody (N term)
Catalog Number: RDI-ALS24201 $312.00/vial $250.00/vial 5+
Quantity: 100ug in 0.1ml (1mg/ml) PBS containing 0.02% sodium azide.
Bulk: cat#RDI-ALS24201X1 $1875.00/1mg
Description: Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B-cell signaling by augmenting B-cell aggregation and survival and down-regulating CD23 expression. CD72 is a lymphocyte receptor for the Class IV semaphorin CD100, which is involved in regulating B cell signaling.
Host Species: Rabbit
Specificity: Reacts with residues 147-158 [GKNEDGKGRCPF] of the human CD100 protein.
Applications: Western blot - Use at 1:500 to 1:1,000 dilution.
Format: Purified IgG
Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.
Reference: Hall et al. (1996).Proc Natl Acad Sci U S A 93(21):11780-5