RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN TYPE I & II

Mab to Cytokeratin Type I & II cat#RDI-PRO61835 $438.00/vial

Clone Determination AE 1 / AE 3

Category Mouse biclonal

Immunoglobulin Class IgG1

Purification Ion-exchange chromatography

Antigen Human epidermal keratin

Specificity AE 1/AE 3 represents an excellent marker with a very broad

range; for distinguishing Ca. from non-epithelial tumors

Application Suitable for frozen and paraffin-embedded tissue (P)

cytological material immunoblotting (Western)

Storage At 4°C one year

Quantity 200 µl (1 mg/ml)

Polypeptide Reacting MR 40 000, MR 48 000 and MR 50 000 polypeptides of human epithelial proteins (cytokeratins nos. 19, 16, 14) and most other acidic (type I) cytokeratins (AE1). Most basic (type II) cytokeratins (MR 52 500 - 68 000) of human epithelia (AE3)

Antigen Recognized in

Species (tested so far) Human, rabbit, mouse, rat, bovine, chick, monkey, guinea pig

Reactivities on Cultured

Cell Lines (tested so far) MCF-7, A-431, RT 112, HeLa

Tumors Specifically Detected All epithelium and their neoplasms

Pretreatment With paraffin-embedded sections, protease pretreatment is recommended prior to antibody application

Working Dilution Dilute 1:200 in PBS pH 7.4 for use in immunohistochemistry Incubation Time 2h at 37°C (or over night at 4°C)

References

Battifora, H., Sheibani, K., Tubbs, R.R., Kopinski, M.I. and Sun, T.-T.: Anti-keratin antibodies in tumor diagnosis: Distinction between seminoma and embryonal carcinoma. Cancer 54, 843-848 (1984)

Eichner, R., Bonitz, P. and Sun, T.-T.: Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J. Cell. Biol. 98, 1388-1396 (1984)

Garin-Chesa, P., Rettig, J. and Melamed, R.: Expression of cyto- keratin in normal and neoplastic colonic epithelial cells: Impli- cations for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829-835 (1986)

Nelson, W.G., Battifora, H., Santana, H. and Sun, T.-T.: Specific keratins as molecular markers for neoplasms with a stratified epithelial origin. Cancer Res. 44, 1600-1603 (1984)

Nelson, W.G. and Sun, T.-T.: The 50- and 58- kD keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244-251 (1983)

Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)

Shi, S.-R., Goodman, M.L., Chan, A.K., Pilch, B.Z., Chen, L.B. and Sun, T.-T.: Immunohistochemical study of nasopharyngeal carcinoma using monoclonal anti-keratin antibodies. Am. J. Pathol. 117, 53-63 (1984)

Spagnolo, D.V., Michie, S.A., Crabtree, G.S., Warnke, R.A. and Rouse, R.V.: Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Pathol. 84, 697-704 (1985)

Sun, T.-T., Eichner, R., Cooper, D., Schermer, A., Nelson, W.G. and Weiss, R.A.: Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. The Cancer Cell 1, 169-176 (1984)

Sun, T.-T., Eichner, R., A., Nelson, W.G. Tseng, S.C.G., Weiss, R.A., Jarvinen, M. and Woodcock-Mitchell, J.: Keratin classes: Molecular markers for different types of epithelial differentiation. J. Invest. Dermatol. 81, 109-115 (1983)

Sun, T.-T., Tseng, S.C.G., Huang, A.J.W., Cooper, D., Lynch, M.H., Weiss, R.A., Eichner, R., A. and Schermer, A.: Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments. The New York Acad. Sci. (E. Wang, D. Fischmann, R. Liem, and T.-T. Sun, eds.) 455, 307-329 (1985)

Tseng, S.C.G., Jarvinen, M., Nelson, W.G., Huang, J.-W., Woodcock-Mitchell, J. and Sun, T.-T.: Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361-372 (1982)

Woodcock-Mitchell, J., Eichner, R., Nelson, W.G. and Sun, T.-T.: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J. Cell Biol. 95, 580-588 (1982)

Weiss, R.A., Eichner, R. and Sun, T.-T.: Monoclonal antibody analyis of keratin expression in epidermal diseases: A 48 kD and a 56 kD keratin as molecular markers for hyperproliferating keratinocytes. J. Cell Biol. 98, 1397-1406 (1984)

Weiss, R.A., Guillet, G.Y., Freedberg, I.M., Small, E.-A., Farmer, E.R., Weiss, M. and Sun, T.-T.: The use of monoclonal antibody to keratin in human epidermal disease: Alterations in immunohistoche- mical staining pattern. J. Invest. Dermatol. 81, 2279-2286 1983.