RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN TYPE 18

cat#RDI-PRO61028 $375.00/vial

also available Biotin conjugated: cat#RDI-PRO61528 $438.00/vial 0.25ml

FITC conjugated: cat#RDI-PRO61428 $438.00/0.25ml

Clone Determination Ks 18.04

Category Mouse monoclonal

Immunoglobulin Class IgG1

Purification Protein A (recombinant)

Antigen Human Cytokeratin 18 from HeLa cytoskeletal preparation

Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin

Application Suitable for frozen and parrafin embeded tissue Cytological material

Immunoblotting (Western)

(micorwave release recommended for paraffin)

Storage At 2-8 DEG C one year after reconstitution

Quantity 50 µg (lyoph.) ; contains 0.5% BSA and 0.09% NaN3

Polypeptide Reacting Mr 45 000 polypeptide ( human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia

Antigen Recognized in Human, mouse, rat, bovine, pig, dog,

Species (tested so far) hamster, sheep, fish (trout)

Reactivities on Cultured HeLa and MCF-7

Cell Lines (tested so far)

Tumors Specifically Detected All tested adeno Ca.; mammary CA, urinary bladder CA, undiff CA, cervix CA,

h epatocellular CA.

Working Dilution After reconstitution with 1 ml dist. water, dilute 1:20 with PBS, pH 7.4 for use in

i mmunohistochemistry

Incubation Time 1 h at 37 DEG C (extended with paraffin (suggest overnight at 2-8 DEG C)

References:

Bartek,J., Vojtesek,B., Staskova,Z., Bartkova,J.,Kerekes,Z. Rejthar,A. and Kovarik,J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology.J. Pathol.(1991) in press.

Heid,H., Vojtesek,B., Bruder,G., Kaufman, M., Stackova,Z., Kovarik,J.,and Franke W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991), submitted.

Layerova,L.,Kovarik,J.,Bartek,J.,Rejthar,A. and Vojtesk, B.: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495-504 (1988).

Markl,J., Winter,S. and Franke,W.W." The catalog and the expression complexity oof cytokeratins in a lower verte-brate: Biochemical identification of cytokerations in a teleost fish, the rainbow trout. Eur.J.Cell Ciol.50, 1-16 (1989).

Moll,R., Franke, W.W., Schiller, D.L.,Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells 31, 11-24 (1982).

Gomi,H.,Yokoyama,T., Fujmoto,K., Ikeda,T., Katoh,A.,Itoh, T, and Itohara,S.: Mice Devoid of the Glial Fibrillary Acidic Protein Develop Normally and Are Susceptible to Scrapie Prions. Neuron,Vol. 14,29-41 (1995).

also available as 5ml TCS cat#RDI-PRO65028 $219.00/vial.