RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 7 see also other cytokeratin 7 abs from main page
Mab to Cytokeratin 7
cat#RDI-PRO10522 $344.00
Clone RCK 105
Category Mouse monoclonal, culture supernatant
IgG Class IgG1
Antigen Isolated From Cell line T24
Clinical Application/ Specificity Specific subtypes of adenocarcinoma. RCK 105 decorates specific subtypes of adenocarcinoma. Cytokeratin 7 was found to occur in the columnar and glandular epithelium of the lung, cervix, breast, bile ducts, larger collecting ducts of the kidney and in mesothelium, but is absent from gastrointestinal epithelium, hepatocytses and myoepithelium. Several types of adenocarcinomas of the mammary gland, ovary and lung are positive. In immunoblotting reactive with the 54kD cytokeratin 7 protein.
Recommended Positive Cotnrol: Glandular epithelium breast
Application Immunoblotting
Immunohistochemistry
Suitable for frozen tissue
Cell Suspensions
Antigen Recognized in species (tested so far) Human, hamster, mouse, rat, dog, pig
Working Dilution 1:5 - 1:10
Dilution Buffer PBS with 1% BSA and 0.09% Na-Azide
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 1 ml
References
Ramaekers F., et al. Tissue distribution of Keratin 7 as monitored by a Monoclonal Antibody (1987). Exp Cell Res 170:235-249.
Fischer, H.P., et al. Keratin Polypeptides in Malignant Epithelial Liver Tumours (1987). Am Journ of Path 127:530-537.
Asborn, M., et al. Differential Diagnosis of Gastro-intestinal Carcinomas by Using Monoclonal Antibodies Specific for Individual Keratin Polypeptides (1986). Lab Invest 55:497-504.
Ramaekers F., et al. Use of Monoclonal Antibodies to Keratin 7 in the Differential Diagnosis of Adenocarcinomas (1990). Am J of Path 136:641-655.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures.