Product Description
Rat VIP(Vasoactive Intestinal Peptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 2.58 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vasoactive Intestinal Peptide(VIP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasoactive Intestinal Peptide(VIP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasoactive Intestinal Peptide(VIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
