Product Description
Rat UTS2R(Urotensin 2 Receptor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: UT; UTS2-R; GPR14; UTR2; Urotensin-II Receptor; Hypocretin Receptor; G Protein-Coupled Receptor 14
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.108 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Endocrinology;Cardiovascular biology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urotensin 2 Receptor(UTS2R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urotensin 2 Receptor(UTS2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urotensin 2 Receptor(UTS2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urotensin 2 Receptor(UTS2R) in the samples is then determined by comparing the OD of the samples to the standard curve.
