Product Description
Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 2.43 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone(TRH) in the samples is then determined by comparing the OD of the samples to the standard curve.
