Rat SOD4(Superoxide Dismutase, Copper Chaperone) ELISA Kit

Product Description

Rat SOD4(Superoxide Dismutase, Copper Chaperone) ELISA Kit is availabe at Gentaur for next week delivery.

Alternative Names:  CCS; Copper Chaperone For Superoxide Dismutase

Species:  Rat

Assay Type:  Sandwich

Sensitivity:  0.055 ng/mL

Standard:  10 ng/mL

Detection Range:  0.16-10 ng/mL

Sample Type:  tissue homogenates, cell lysates and other biological fluids

Assay Length:  4.5h

Research AreaResearch Area:  Enzyme & Kinase;Metabolic pathway;Developmental science;

Test Principle:  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase, Copper Chaperone(SOD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase, Copper Chaperone(SOD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase, Copper Chaperone(SOD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase, Copper Chaperone(SOD4) in the samples is then determined by comparing the OD of the samples to the standard curve.