Product Description
Rat S1PR3(Sphingosine 1 Phosphate Receptor 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: EDG3; LPB3; S1P3; S1PR3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; Sphingosine 1-phosphate receptor Edg-3
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.237 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3(S1PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3(S1PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 3(S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 3(S1PR3) in the samples is then determined by comparing the OD of the samples to the standard curve.
