Product Description
Rat ROS1(C-Ros Oncogene 1, Receptor Tyrosine Kinase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 6.6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase(ROS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase(ROS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase(ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase(ROS1) in the samples is then determined by comparing the OD of the samples to the standard curve.
