Product Description
Rat PSGL1(P-Selectin Glycoprotein Ligand 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD162; SELPLG; CLA; Selectin P Ligand
Species: Rat
Assay Type: Sandwich
Sensitivity: 28.5 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin Glycoprotein Ligand 1(PSGL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to P-Selectin Glycoprotein Ligand 1(PSGL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P-Selectin Glycoprotein Ligand 1(PSGL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P-Selectin Glycoprotein Ligand 1(PSGL1) in the samples is then determined by comparing the OD of the samples to the standard curve.
