Product Description
Rat Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Rat
Assay Type: Sandwich
Sensitivity: 15.6 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5 pg/mL-4000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pro Atrial Natriuretic Peptide(Pro-ANP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pro Atrial Natriuretic Peptide(Pro-ANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro Atrial Natriuretic Peptide(Pro-ANP) in the samples is then determined by comparing the OD of the samples to the standard curve.
