Product Description
Rat PP(Pancreatic Polypeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 8.85 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pancreatic Polypeptide(PP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreatic Polypeptide(PP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreatic Polypeptide(PP) in the samples is then determined by comparing the OD of the samples to the standard curve.
