Product Description
Rat POMC(Proopiomelanocortin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 52.5 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Proopiomelanocortin(POMC) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proopiomelanocortin(POMC). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proopiomelanocortin(POMC) in the samples is then determined by comparing the OD of the samples to the standard curve.
