Product Description
Rat PKBb(Protein Kinase B Beta) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: AKT2; PKBBETA; PRKB-B; RAC-BETA; RAC-Beta Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 2
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Beta(PKBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Beta(PKBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Beta(PKBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Beta(PKBb) in the samples is then determined by comparing the OD of the samples to the standard curve.
