Product Description
Rat PICP(Procollagen I C-Terminal Propeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.54 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Hepatology;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I C-Terminal Propeptide(PICP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen I C-Terminal Propeptide(PICP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I C-Terminal Propeptide(PICP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I C-Terminal Propeptide(PICP) in the samples is then determined by comparing the OD of the samples to the standard curve.
