Rat PDHa(Pyruvate Dehydrogenase Alpha) ELISA Kit

Product Description

Rat PDHa(Pyruvate Dehydrogenase Alpha) ELISA Kit is availabe at Gentaur for next week delivery.

Alternative Names:  PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial

Species:  Rat

Assay Type:  Sandwich

Sensitivity:  0.29 ng/mL

Standard:  50 ng/mL

Detection Range:  0.79-50 ng/mL

Sample Type:  tissue homogenates, cell lysates and other biological fluids

Assay Length:  4.5h

Research AreaResearch Area:  Enzyme & Kinase;Metabolic pathway;

Test Principle:  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Alpha(PDHa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Alpha(PDHa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Alpha(PDHa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Alpha(PDHa) in the samples is then determined by comparing the OD of the samples to the standard curve.