Product Description
Rat PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Plasma
Assay Length: 4.5h
Research AreaResearch Area: Hematology;Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 2(PAI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 2(PAI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 2(PAI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasminogen Activator Inhibitor 2(PAI2) in the samples is then determined by comparing the OD of the samples to the standard curve.
