Product Description
Rat NHE3(Sodium/Hydrogen Exchanger 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SLC9A3; Solute Carrier Family 9 Member 3; Sodium-hydrogen antiporter 3; Na(+)/H(+) exchanger 3
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.126 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 3(NHE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 3(NHE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 3(NHE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 3(NHE3) in the samples is then determined by comparing the OD of the samples to the standard curve.
