Product Description
Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 51.7 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurofilament, Light Polypeptide(NEFL) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide(NEFL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament, Light Polypeptide(NEFL) in the samples is then determined by comparing the OD of the samples to the standard curve.
